Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.

Rapid HIV-1 Capsid Interaction Screening Using Fluorescence Fluctuation Spectroscopy

Labeling of virus components for advanced, quantitative imaging analyses - Sakin - 2016 - FEBS Letters - Wiley Online Library

2021 Top 10 Innovations The Scientist Magazine®

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Viruses, Free Full-Text

Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viruses, Free Full-Text

Detecting RNA viruses in living mammalian cells by fluorescence microscopy: Trends in Biotechnology

Altered host protease determinants for SARS-CoV-2 Omicron

Nonclinical pharmacokinetics and biodistribution of VSV-GP using methods to decouple input drug disposition and viral replication: Molecular Therapy - Methods & Clinical Development

Dynamic Oligomerization of Integrase Orchestrates HIV Nuclear Entry

Biosensors, Free Full-Text

Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes

Viruses, Free Full-Text